Furthermore, a resolution for separation of IgG and BSA by MPR Q was 1.06 at 5.09 cm/min, while it was higher than that for the conventional resin at all linear velocities from 5.09 cm/min to 50.93 cm/min. It combines high capacity with high ow velocity and low backpressure, at the same time reducing process cycle times and increasing productivity. The dynamic binding capacity at 10% breakthrough of MPR Q, determined by frontal analysis, exhibited a capacity of 43.8 mg/cm 3 at 5.09 cm/min and 58% of DBC 10% was maintained even though the linear velocity was increased to 50.93 cm/min. Capto Q is a strong anion exchange medium for packed bed chromatography that allows increased speed and throughput in capture and intermediate puri cation (Fig 1). Strong anion exchange resins (Toyopearl Gigacap Q-650M, Capto Q, TSKgel SuperQ. It is composed of a rigid, high-flow agarose matrix modified with dextran surface extenders and a strong quaternary ammonium (Q) anion exchanger. SuperQ-5PW and Toyopearl GigaCap Q-650M resins (20m and 75m) gave. Moreover, the reduced height equivalent to a theoretical plate, h, of human IgG, determined using the linear gradient elution method was 65.8 and was not significantly changed in the range of linear velocities from 20.37 to 50.93 cm/min. Offers increased productivity Capto Q is designed for capture and intermediate purification of proteins from large feed volumes. Inverse size-exclusion chromatography (iSEC) experiments revealed that the apparent pore diameter of MPR Q was approximately 90 nm, which was sufficiently large for the penetration of human IgG and bovine IgM. Under static conditions, MPR Q exhibited a binding capacity of 49.8 mg-IgG/cm 3-resin at pH 10, whereas the fastest adsorption was observed among the anion-exchanger resins tested. Safety For use and handling of the products in a safe way, refer to the Safety Data Sheet. MPR Q resin was prepared by phase separation based on spinodal decomposition followed by dextran grafting and ligand conjugation. Capto HiRes Q 5/50, Capto HiRes Q 10/100, Capto HiRes S 5/50, and Capto HiRes S 10/100 columns are intended for research use only and shall not be used in any clinical or in vitro procedures for diagnostic purposes. This study investigated the adsorption capacity and mass transfer properties of a novel macroporous epoxy-polymer-based anion-exchanger, MPR Q, for the efficient separation of therapeutic proteins.
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